The Exon 6ABC Region of Amelogenin mRNA Contribute to Increased Levels of Amelogenin mRNA through Amelogenin Protein-enhanced mRNA Stabilization
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چکیده
منابع مشابه
Heterogeneity of amelogenin mRNA in the bovine tooth germ.
The amelogenins are a complex mixture of hydrophobic proteins that are the major organic component of developing enamel. To study the molecular mechanisms underlying the heterogeneity of the amelogenins we isolated cDNA clones encoding these proteins. The clones were definitively identified by hybrid-selected translation experiments and by comparison of the DNA sequence with the protein-derived...
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How did we humans (and other vertebrates) get our sparkling smiles? The hard white coating on our teeth, the enamel, is made up of hydroxyapatite—the same mineral that forms our bones. In our teeth, hydroxyapatite is organized into parallel arrays of columnar apatite crystals called prisms. The growth and organization of enamel prisms is controlled by special proteins secreted by cells known as...
متن کاملAmelogenin is a cell adhesion protein.
Amelogenin, the major protein component of tooth enamel, is shown to be a cell adhesion protein. Since it had been shown that an amelogenin-containing preparation, Emdogain, possessed cell-adhesive activity, we tested the hypothesis that amelogenin was responsible for cell-adhesive activity. Recombinant amelogenin was found to promote adhesion at less than 15 micro g/60-mm plate and requires di...
متن کاملOne-Step Purification of Recombinant Human Amelogenin and Use of Amelogenin as a Fusion Partner
Amelogenin is an extracellular protein first identified as a matrix component important for formation of dental enamel during tooth development. Lately, amelogenin has also been found to have positive effects on clinical important areas, such as treatment of periodontal defects, wound healing, and bone regeneration. Here we present a simple method for purification of recombinant human amelogeni...
متن کاملQuantitative analysis of amelogenin solubility.
Amelogenins are a group of extracellular enamel matrix proteins which are believed to be involved in the regulation of the size and habits of forming enamel crystals. The aim of this study was to compare the solubility properties of several amelogenins at various pH (from 4.0 to 9.0) at constant ionic strength (IS), and to examine the influence of buffer composition, IS, and divalent metal ions...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2006
ISSN: 0021-9258
DOI: 10.1074/jbc.m605406200